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    [–] Scientists develop CRISPR-SKIP, a new CRISPR technique that skips over portions of genes that can cause disease, instead of turning them off by breaking the DNA. Such targeted editing could one day treat genetic diseases such as Duchenne’s muscular dystrophy, Huntington’s disease or some cancers. mvea 11 points ago in science

    The title of the post is a copy and paste from the title, second and third paragraphs of the linked academic press release here :

    New CRISPR technique skips over portions of genes that can cause disease

    Such targeted editing could one day be useful for treating genetic diseases caused by mutations in the genome, such as Duchenne’s muscular dystrophy, Huntington’s disease or some cancers.

    CRISPR technologies typically turn off genes by breaking the DNA at the start of a targeted gene, inducing mutations when the DNA binds back together.

    Journal Reference:

    Michael Gapinske, Alan Luu, Jackson Winter, Wendy S. Woods, Kurt A. Kostan, Nikhil Shiva, Jun S. Song, Pablo Perez-Pinera.

    CRISPR-SKIP: programmable gene splicing with single base editors.

    Genome Biology, 2018; 19 (1)

    DOI: 10.1186/s13059-018-1482-5

    Link: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1482-5

    Abstract:

    CRISPR gene editing has revolutionized biomedicine and biotechnology by providing a simple means to engineer genes through targeted double-strand breaks in the genomic DNA of living cells. However, given the stochasticity of cellular DNA repair mechanisms and the potential for off-target mutations, technologies capable of introducing targeted changes with increased precision, such as single-base editors, are preferred. We present a versatile method termed CRISPR-SKIP that utilizes cytidine deaminase single-base editors to program exon skipping by mutating target DNA bases within splice acceptor sites. Given its simplicity and precision, CRISPR-SKIP will be broadly applicable in gene therapy and synthetic biology.

    [–] Scientists develop CRISPR-SKIP, a new CRISPR technique that skips over portions of genes that can cause disease, instead of turning them off by breaking the DNA. Such targeted editing could one day treat genetic diseases such as Duchenne’s muscular dystrophy, Huntington’s disease or some cancers. mvea 1 points ago in Futurology

    The title of the post is a copy and paste from the title, second and third paragraphs of the linked academic press release here :

    New CRISPR technique skips over portions of genes that can cause disease

    Such targeted editing could one day be useful for treating genetic diseases caused by mutations in the genome, such as Duchenne’s muscular dystrophy, Huntington’s disease or some cancers.

    CRISPR technologies typically turn off genes by breaking the DNA at the start of a targeted gene, inducing mutations when the DNA binds back together.

    Journal Reference:

    Michael Gapinske, Alan Luu, Jackson Winter, Wendy S. Woods, Kurt A. Kostan, Nikhil Shiva, Jun S. Song, Pablo Perez-Pinera.

    CRISPR-SKIP: programmable gene splicing with single base editors.

    Genome Biology, 2018; 19 (1)

    DOI: 10.1186/s13059-018-1482-5

    Link: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1482-5

    Abstract:

    CRISPR gene editing has revolutionized biomedicine and biotechnology by providing a simple means to engineer genes through targeted double-strand breaks in the genomic DNA of living cells. However, given the stochasticity of cellular DNA repair mechanisms and the potential for off-target mutations, technologies capable of introducing targeted changes with increased precision, such as single-base editors, are preferred. We present a versatile method termed CRISPR-SKIP that utilizes cytidine deaminase single-base editors to program exon skipping by mutating target DNA bases within splice acceptor sites. Given its simplicity and precision, CRISPR-SKIP will be broadly applicable in gene therapy and synthetic biology.

    [–] Researchers have developed the world's first-ever 4D printing for ceramics that are mechanically robust and can have complex shapes. 4D printing is 3D printing combined with time as the 4th dimension, where the printed objects can re-shape or self-assemble themselves over time with external stimuli. mvea 1 points ago in science

    The title of the post is a copy and paste from the first and fifth paragraphs of the linked academic press release here :

    Chinese researchers have developed the world's first-ever 4D printing for ceramics that are mechanically robust and can have complex shapes, offering broad potential applications in telecommunications, electronics and even space exploration.

    4D printing is conventional 3D printing combined with the additional element of time as the fourth dimension, where the printed objects can re-shape or self-assemble themselves over time with external stimuli, such as mechanical force, temperature, or a magnetic field.

    Journal Reference:

    Guo Liu, Yan Zhao, Ge Wu, Jian Lu.

    Origami and 4D printing of elastomer-derived ceramic structures.

    Science Advances, 2018; 4 (8): eaat0641

    DOI: 10.1126/sciadv.aat0641

    Link: http://advances.sciencemag.org/content/4/8/eaat0641

    Abstract

    Four-dimensional (4D) printing involves conventional 3D printing followed by a shape-morphing step. It enables more complex shapes to be created than is possible with conventional 3D printing. However, 3D-printed ceramic precursors are usually difficult to be deformed, hindering the development of 4D printing for ceramics. To overcome this limitation, we developed elastomeric poly(dimethylsiloxane) matrix nanocomposites (NCs) that can be printed, deformed, and then transformed into silicon oxycarbide matrix NCs, making the growth of complex ceramic origami and 4D-printed ceramic structures possible. In addition, the printed ceramic precursors are soft and can be stretched beyond three times their initial length. Hierarchical elastomer-derived ceramics (EDCs) could be achieved with programmable architectures spanning three orders of magnitude, from 200 μm to 10 cm. A compressive strength of 547 MPa is achieved on the microlattice at 1.6 g cm−3. This work starts a new chapter of printing high-resolution complex and mechanically robust ceramics, and this origami and 4D printing of ceramics is cost-efficient in terms of time due to geometrical flexibility of precursors. With the versatile shape-morphing capability of elastomers, this work on origami and 4D printing of EDCs could lead to structural applications of autonomous morphing structures, aerospace propulsion components, space exploration, electronic devices, and high-temperature microelectromechanical systems.

    [–] Researchers have developed the world's first-ever 4D printing for ceramics that are mechanically robust and can have complex shapes. 4D printing is 3D printing combined with time as the 4th dimension, where the printed objects can re-shape or self-assemble themselves over time with external stimuli. mvea 2 points ago in Futurology

    The title of the post is a copy and paste from the first and fifth paragraphs of the linked academic press release here :

    Chinese researchers have developed the world's first-ever 4D printing for ceramics that are mechanically robust and can have complex shapes, offering broad potential applications in telecommunications, electronics and even space exploration.

    4D printing is conventional 3D printing combined with the additional element of time as the fourth dimension, where the printed objects can re-shape or self-assemble themselves over time with external stimuli, such as mechanical force, temperature, or a magnetic field.

    Journal Reference:

    Guo Liu, Yan Zhao, Ge Wu, Jian Lu.

    Origami and 4D printing of elastomer-derived ceramic structures.

    Science Advances, 2018; 4 (8): eaat0641

    DOI: 10.1126/sciadv.aat0641

    Link: http://advances.sciencemag.org/content/4/8/eaat0641

    Abstract

    Four-dimensional (4D) printing involves conventional 3D printing followed by a shape-morphing step. It enables more complex shapes to be created than is possible with conventional 3D printing. However, 3D-printed ceramic precursors are usually difficult to be deformed, hindering the development of 4D printing for ceramics. To overcome this limitation, we developed elastomeric poly(dimethylsiloxane) matrix nanocomposites (NCs) that can be printed, deformed, and then transformed into silicon oxycarbide matrix NCs, making the growth of complex ceramic origami and 4D-printed ceramic structures possible. In addition, the printed ceramic precursors are soft and can be stretched beyond three times their initial length. Hierarchical elastomer-derived ceramics (EDCs) could be achieved with programmable architectures spanning three orders of magnitude, from 200 μm to 10 cm. A compressive strength of 547 MPa is achieved on the microlattice at 1.6 g cm−3. This work starts a new chapter of printing high-resolution complex and mechanically robust ceramics, and this origami and 4D printing of ceramics is cost-efficient in terms of time due to geometrical flexibility of precursors. With the versatile shape-morphing capability of elastomers, this work on origami and 4D printing of EDCs could lead to structural applications of autonomous morphing structures, aerospace propulsion components, space exploration, electronic devices, and high-temperature microelectromechanical systems.